Improvements
Added new options
--pbmm2
and--pbmm2-unmapped
that include additional processing to match the output of PacBio's pbmm2.Added ability to use unaligned BAM as input instead of FASTQ (
--in-bam
).A new higher compression ratio option for BAM/CRAM files with
--gpuwrite
.The nvCOMP DEFLATE compression algorithm options have changed for the parameter
--gpuwrite-deflate-algo
. Please see--help
or the documentation on fq2bam for more details.Added GPU acceleration for CRAM write operations (
--gpuwrite
).
fq2bam has been surpassed by fq2bamfast: the new version of fq2bam with more optimizations for performance. Note that fq2bamfast is no longer present as the fq2bam and fq2bamfast tools have been merged.
Added a new option to use a single-ended unaligned BAM as input instead of a FASTQ file (
--in-se-bam
).A new higher compression ratio option for BAM/CRAM files with
--gpuwrite
.The nvCOMP DEFLATE compression algorithm options have changed for the parameter
--gpuwrite-deflate-algo
. Please see--help
or the documentation on fq2bam for more details.Added GPU acceleration for CRAM write operations (
--gpuwrite
).Default
--min-read-length
is 1 base pair; whereas, in fq2bamfast it was 10.Improved error messaging if paired reads have different names.
Added a new option
--filter-reads-too-long
to filter out reads with length > 512bp.Improved performance and reduced memory consumption when processing long reads (PacBio and ONT data)
haplotypecaller added support for:
Force-calling mode (
--htvc-alleles
).The
--min-base-quality-score
argument.The
--adaptive-pruning
argument.A new annotation
AssemblyComplexity
can be specified by passing--haplotypecaller-options '-A AssemblyComplexity'
.
Added more sanity checks to handle possible errors in advance.
germline pipeline and deepvariant_germline:
Grace Hopper (GH200)-specific optimizations.
Default
--min-read-length
is now 1 base pair to be in line with fq2bam.Improved error messaging if paired reads have different names.