Groups reads together that appear to have come from the same original molecule.


$ pbrun groupreadsbyumi \ --ref Homo_sapiens_assembly38.fasta \ --in-bam input.bam \ --out-bam output.bam

Groups reads together that appear to have come from the same original molecule.

Input/Output file options

--in-bam IN_BAM

Path to the input BAM file. (default: None)

Option is required.

--out-bam OUT_BAM

Path of output BAM file after grouping reads. (default: None)

Option is required.

--ref REF

Path to the reference file. (default: None)

Option is required.

Options specific to this tool

--strategy STRATEGY

The UMI assignment strategy: adjacency or paired. (default: )

Option is required.

--num-zip-threads NUM_ZIP_THREADS

Number of CPUs to use for zipping bam files in a run (default 16 for coordinate sorts and 10 otherwise) (default: None)

--num-sort-threads NUM_SORT_THREADS

Number of CPUs to use for sorting in a run (default 10 for coordinate sorts and 16 otherwise) (default: None)

--max-records-in-ram MAX_RECORDS_IN_RAM

Maximum number of records in RAM when using a queryname or template coordinate sort mode; lowering this number will decrease maximum memory usage. (default: 65000000)

Common options:

--logfile LOGFILE

Path to the log file. If not specified, messages will only be written to the standard error output. (default: None)

--tmp-dir TMP_DIR

Full path to the directory where temporary files will be stored.

--with-petagene-dir WITH_PETAGENE_DIR

Full path to the PetaGene installation directory. By default, this should have been installed at /opt/petagene. Use of this option also requires that the PetaLink library has been preloaded by setting the LD_PRELOAD environment variable. Optionally set the PETASUITE_REFPATH and PGCLOUD_CREDPATH environment variables that are used for data and credentials (default: None)


Do not delete the directory storing temporary files after completion.

--license-file LICENSE_FILE

Path to license file license.bin if not in the installation directory.


Do not override seccomp options for docker (default: None).


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© Copyright 2022, Nvidia. Last updated on Jun 28, 2023.