# Tutorials

The tutorials walk you through a simple use case of Clara Parabricks, giving you a brief introduction to how it works.

• reference file (Homo_sapiens_assembly38.fasta) and its index

• A 'known indels' file and its index

• Two FASTQ files

• Associated index files

The tutorials then walk you through the following steps:

• Alignment (FASTA + FASTQ ==> BAM)

• Variant calling (BAM ==> VCF)

• Computation of BAM and VCF metrics

The tutorials are meant to be simple and straightforward, and to only cover a single, specific use case. You should be able to copy and paste the commands into a terminal window and get the same results as shown. The How-Tos cover more general problem solving using Clara Parabricks.

Steps in the Tutorial