Clara Parabricks v3.8.0
v3.8.0

lofreq

Call variants with high sensitivity, predicting variants below the average base-call quality (Original Lofreq Project). The call part is accelerated.

Copy
Copied!
            

$ pbrun lofreq \ --ref Ref/Homo_sapiens_assembly38.fasta \ --in-tumor-bam tumor.bam \ --in-normal-bam normal.bam \ --output-dir output

Note that the value for --output-dir will be used as a prefix for all the output filenames. If you want the output files to be placed in a folder called output, use --output-dir output/ (note the trailing slash). You'll also need to create the directory before running lofreq.

The command below is the original CPU counterpart of the Parabricks command above. The output from this command will be identical to the output from the above command. See the Output Comparison page for comparing the results.

Copy
Copied!
            

lofreq somatic -n normal.bam -t tumor.bam \ -o output -f /data/Ref/GRCh38.d1.vd1.fa \ --baq-off --no-src-qual --call-rlx-extra-args "@d 2147483647"

Call variants with high sensitivity, predicting variants below the average base-call quality.

Input/Output file options

--ref REF

Path to the reference file. (default: None)

Option is required.

--in-tumor-bam IN_TUMOR_BAM

Path of the BAM/CRAM file for tumor reads. This option is required. (default: None)

Option is required.

--in-normal-bam IN_NORMAL_BAM

Path of the BAM/CRAM file for normal reads. This option is required. (default: None)

Option is required.

--in-dbsnp-file IN_DBSNP_FILE

Path to an input DBSNP file containing known germline variants. Must be in vcf.gz format with its tabix index. (default: None)

--ignore-vcf IGNORE_VCF

Path to an input VCF file containing variants that will be ignored for source quality computation in tumor. If this option is not used, stringently filtered predictions in normal sample will be used by default. (default: None)

--output-dir OUTPUT_DIR

Path to the directory that will contain all of the generated files. (default: None)

Option is required.

Tool Options:

--out-prefix OUT_PREFIX

Prefix filename for output data. (default: None)

--num-threads NUM_THREADS

Number of threads per GPU for each call. (default: 4)

--tumor-mtc TUMOR_MTC

Type of multiple testing correction for tumor. Possible values are {bonf,holm-bonf,fdr}. Default value is bonf. (default: bonf)

--tumor-mtc-alpha TUMOR_MTC_ALPHA

Multiple testing correction alpha for tumor. Default value is 1.000000. (default: 1.0)

--min-cov MIN_COV

Minimum coverage for somatic calls. (default: 7)

--germline

Also list germline calls in separate file. (default: None)

--use-orphan

Use orphaned samples. (default: None)

--baq-on

Use BAQ (base-call quality correction) in all samples. (default: None)

--src-qual

Use source quality in tumor. (default: None)

Common options:

--logfile LOGFILE

Path to the log file. If not specified, messages will only be written to the standard error output. (default: None)

--tmp-dir TMP_DIR

Full path to the directory where temporary files will be stored.

--with-petagene-dir WITH_PETAGENE_DIR

Full path to the PetaGene installation directory. By default, this should have been installed at /opt/petagene. Use of this option also requires that the PetaLink library has been preloaded by setting the LD_PRELOAD environment variable. Optionally set the PETASUITE_REFPATH and PGCLOUD_CREDPATH environment variables that are used for data and credentials (default: None)

--keep-tmp

Do not delete the directory storing temporary files after completion.

--license-file LICENSE_FILE

Path to license file license.bin if not in the installation directory.

--no-seccomp-override

Do not override seccomp options for docker (default: None).

--version

View compatible software versions.

GPU options:

--num-gpus NUM_GPUS

Number of GPUs to use for a run. GPUs 0..(NUM_GPUS-1) will be used.

--gpu-devices GPU_DEVICES

GPU devices to use for a run. By default, all GPU devices will be used. To use specific GPU devices, enter a comma-separated list of GPU device numbers. Possible device numbers can be found by examining the output of the nvidia-smi command. For example, using --gpu-devices 0,1 would only use the first two GPUs.

© Copyright 2023, Nvidia. Last updated on Jun 28, 2023.